C2441-06M
CD90 (Thy-1) (PE)
Size
£723.00
- SKU:
- C2441-06M
- Application:
- Flow Cytometry
- Purity:
- Purified by Ion Exchange chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat Thy-1
- Clone:
- 3H1751 (OX-7)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 1% BSA, 5% sucrose, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- The antibody is against the Thy-1.1 antigenic determinant which is a monomorphic determinant within rat strains but polymorphic in the mouse. Thus 3H1751 reacts with Thy-1.1 mice (eg AKR strain) but not Thy-1.2 mice (eg CBA, BALB/c). The affinity of the Fab of 3H1751 for rat Thy-1 is 3x10e9M-1 and for mouse Thy-1.1 is 3x10e8M-1 (1). The Thy-1 antigen is found on a variety of cell types including thymocytes, neuronal cells, T lymphocytes (mouse), stem cells and immature B cells (rat) and connective tissue. The molecule is a glycoprotein with 112 amino acids which is homologous to immunoglobulin domains (3). The binding characteristics of 3H1751 have been extensively studied (1) and this is an excellent antibody for studying the killing of Thy-1+ tumor cells with antibody-toxin conjugates (4). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, 10ul labels 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Hybridoma: NS1myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice