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C2441-06F

CD90 (Thy-1) (FITC)

Size

£620.00

SKU:
C2441-06F
Application:
Flow Cytometry
Purity:
Purified by Protein G affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Purified human brain Thy-1.
Clone:
3H1746 (F15-42-1)
Format:
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:
CD90 (25kD glycoprotein) is expressed by a subset of CD34-positive cells in the bone marrow and by prothymocytes within the thymus. CD90 is also extensively expressed within the brain. Human CD90 is homologous to rat Thy1. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Hybridoma: Spleen cells from immunized BALB/c mice fused with mouse NS-1 myeloma cells. Recommended Negative Controls: I1904-78R: IgG1 Murine Negative Control I1904-78R3 IgG1 Murine Negative Control (FITC) Recommended Secondary Antibodies: Rat Adsorbed I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice