C2438-23E
CD86 (Activation B7-2 Antigen, B70, B7-2, BU63, CD28LG2, CTLA-4 Counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte Activation Antigen CD86 Precursor) (APC)
Size
£486.00
- SKU:
- C2438-23E
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- LPS-activated mouse B cells
- Clone:
- GL1
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with Allophycocyanin (APC).
- Extra Details:
- Mouse CD86, the B7-2 co-stimulatory molecule and a ligand for CD28 and CD152/CTLA-4 CD86, also known as B7-2, is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes.1-5 CD86 expression is rapidly upregulated by B-cell specific stimuli with peak expression at 18-42 hours after stimulation.1, 5 CD86, along with CD80/B7-1, is an important accessory molecule in T cell costimulation via its interaction with CD28 and CD152/CTLA-4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response.1-9 This antibody blocks mixed lymphocyte reactions and stimulation of T cells by antigen-presenting cells.1, 3, 5 Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤ 0.1ug/10e6 cells in final volume of 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice