C2436-10C
CD84 (FITC)
Size
£704.00
- SKU:
- C2436-10C
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human MLR stimulated lymphocytes
- Clone:
- 7H106 (152.1D5)
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.05% sodium azide, 5% glycerol. Labeled with fluorescein isothiocyanate (FITC).
- Extra Details:
- Human CD84 is a cell surface glycoprotein found on mature B lymphocytes, monocytes, and a subset of T lymphocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 (10ug/ml). 80ul labels 5x10e5 Raji cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured Raji cells were incubated 45 minutes on ice with 80ul of C2436-10C at a 1:50 dilution (10ug/ml). 2. They were then washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.81 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD84 antibody (C2436-10A). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice