C2436-10A
CD84
Size
£557.00
- SKU:
- C2436-10A
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human MLR stimulated lymphocytes
- Clone:
- 7H106 (152.1D5)
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human CD84 is a cell surface glycoprotein found on mature B lymphocytes, monocytes, and a subset of T lymphocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml per 5x10e4 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Raji cells were incubated 45 minutes on ice with 80ul of C2436-10A at 5ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) Goat anti-Mouse, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. 3. Cells stained positive with a mean shift of 1.43 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice