C2435-04C
CD83 (PE) (B Cell activation protein, BL11, Cell Surface Protein HB15)
Size
£704.00
- SKU:
- C2435-04C
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- COS cells transfected with human CD83.
- Clone:
- 7H190
- Format:
- Supplied as a liquid in PBS, pH 7.5, 15% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with R-Phycoerythrin (PE)
- Extra Details:
- Human CD83 is a type 1 cell surface glycoprotein expressed almost solely by dendritic cells that play an important role in antigen presentation (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (80ul/test) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 human Raji cells per tube were washed and incubated for 45 minutes on ice with 80ul of C2435-04C at a dilution factor of 1:50. 2. Cells washed three times, fixed and analyzed using a BD FACS Calibur. 3. Cells stained positive with a mean shift of 0.6 log10 fluorescent units when compared to a mouse IgG1/R-PE negative control at a similar concentration. 4. Binding was 99% blocked when cells were pre-incubated for 10 minutes with 20ul of C2435-04 at 0.5mg/ml. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice