C2435-04B
CD83 (B Cell activation protein, BL11, Cell Surface Protein HB15) (FITC)
Size
£557.00
- SKU:
- C2435-04B
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- COS cells transfected with human CD83.
- Clone:
- 7H190
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.05% sodium azide, 5% glycerol. Labeled with FITC.
- Extra Details:
- Human CD83 is a type 1 cell surface glycoprotein expressed almost solely by dendritic cells that play an important role in antigen presentation (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (10ug/ml) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Raji cells were washed and incubated 45 minutes on ice with 80ul of C2435-04B at a 1:50 dilution (10ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.56 log10 fluorescent units when compared to a Mouse IgG1/FITC negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD83 antibody (C2435-04). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice