C2435-04
CD83 (B Cell activation protein, BL11, Cell Surface Protein HB15)
Size
£479.00
- SKU:
- C2435-04
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- COS cells transfected with human CD83
- Clone:
- 7H190
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human CD83 is a type 1 cell surface glycoprotein expressed almost solely by dendritic cells that play an important role in antigen presentation (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml; 80ul labels 5x10e5 Raji cells Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured Raji cells per tube were incubated 45 minutes on ice with 80ul of C2435-04 at 10ug/ml. 2. Cells were washed twice and incubated with 2º reagent Goat anti-Mouse IgG/FITC. 3. Cells were then washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 0.66 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Recommended Secondary Antibodies: I1904-04A: IgG, H&L (FITC) Pab Gt xMo I1904-06F: IgG, H&L (FITC) (X-absorbed) Pab Gt xMo I1904-14A: IgG, Fc (FITC) Pab Gt xMo I1904-25A: IgG H&L (FITC) Pab Gt xMo Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice