C2433-21B

CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (FITC)

Size

£550.00

SKU:: C2433-21B
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Hamster
Immunogen:: Mouse epithelial cell line PAM212
Clone:: 2F7
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: CD81 (Mr 25kD) is a member of the transmembrane 4 integral membrane protein family CD81/TAPA-1 is an integral membrane protein expressed on a variety of cell types, and has a high degree of sequence homology between human and mouse.1 CD81 is expressed on thymic stromal cells where it plays an important role in the transition of gamma/delta+ T cells to more mature T cells with alpha/beta T cell receptors.2 Immunohistochemical staining has revealed that its expression is localized to the subcapsular region of the thymus and, specifically, on cells that have distinct clustering patterns. It has been speculated that the ligand for CD81 is the pre-T cell receptor, which is composed of a TCR beta chain and glycoprotein pTalpha.2 This antibody can block thymocyte interaction with CD81 in vitro.2 Applications • Identification and enumeration of CD81+ cells by flow cytometry 2 • Immunohistochemistry (frozen sections) 2 • Immunoprecipitation 2 • In vitro blocking of thymocyte interactions with CD81 2 Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice