C2433-12A-HRP
CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (HRP)
Size
£837.00
- SKU:
- C2433-12A-HRP
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- 38C13, murine B cell line.
- Clone:
- 3H1702 (Eat 2)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- CD81, also known as TAPA-1 or Target of the antiproliferative antibody, is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. C2433-12A induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maecker et al. 2000). C2433-12A requires the presence of both extracellular loops of TAPA-1 for binding. Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubenstein et al. 2006). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): The recognized epitope is reported to be sensitive to formaldehyde fixation and tissue processing. Use of acetone fixation is recommened. Western Blot: non-reducing conditions Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized Armenian hamsters were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line. Recommended Secondary Reagents: I1903-04T: IgG H&L (HRP) Pab Gt x Hm I1903-04Q: IgG H&L (FITC) Pab Gt x Hm Recommended Negative Controls: Hamster IgG Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice