C2433-12A-BIOTIN
CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (Biotin)
Size
£837.00
- SKU:
- C2433-12A-BIOTIN
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- 38C13, murine B cell line.
- Clone:
- 3H1702 (Eat 2)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- CD81, also known as TAPA-1 or Target of the antiproliferative antibody, is a 236 amino acid ~26 kDa multipass transmembrane protein belonging to the TM4SF family (UniProt: P35762). In rodents CD81 is expressed at much higher levels on resting B cells than on T cells, although increased expression on T cells is found following activation. C2433-12A induces homotypic aggregation of B cells and inhibits anti Ig and IL-4 induced proliferation (Maecker et al. 2000). C2433-12A requires the presence of both extracellular loops of TAPA-1 for binding. Mice lacking CD81 demonstrate reduced fertility through impaired oocyte-sperm fusion, double knockout CD81-/- CD9-/- mice are completely infertile suggesting complimentary roles in oocyte-sperm fusion (Rubenstein et al. 2006). Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): The recognized epitope is reported to be sensitive to formaldehyde fixation and tissue processing. Use of acetone fixation is recommened. Western Blot: non-reducing conditions Optimal dilutions to be determined by the researcher. Recommended Secondary Reagents: I1903-04T: IgG H&L (HRP) Pab Gt x Hm I1903-04Q: IgG H&L (FITC) Pab Gt x Hm Recommended Negative Controls: Hamster IgG Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice