C2433-10B

CD81 (TAPA-1, Target of anti-Proliferative Antibody-1) (Biotin)

Size

£607.00

SKU:: C2433-10B
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human B cell line from Burkitt lymphoma.
Clone:: 7H100
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Extra Details:: Human CD81 is a member of the tetraspan family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion and cellular activation. Association of CD81 with integrins appears to be functionally important in the regulation of cell adhesion and activation. Recent work with CD81-null mice indicates CD81 is important for development of humoral immune response. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml; 80ul labels 5x10e5 cultured human Raji cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured human Raji cells per tube were incubated for 45 minutes on ice with 80ul of C2433-10B at 10ug/ml. 2. Washed cells twice and incubated with S7973-88P: Streptavidin (PE). 3. Washed cells three times, fixed and analyzeed by FACS. 4. Cells stained positive with a mean shift of 2.3 log10 fluorescent units when compared to IgG1 Murine Negative Control (Biotin) at a similar concentration. 5. Binding was blocked when cells were pre-incubated with 20ul of C2433-10A at a concentration of 0.5mg/ml. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice