C2433-10A
CD81 (TAPA-1, Target of anti-Proliferative Antibody-1)
Size
£576.00
- SKU:
- C2433-10A
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human B cell line from Burkitt lymphoma.
- Clone:
- 7H100
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml Gentamicin.
- Extra Details:
- Human CD81 is a member of the tetraspan family of leukocyte cell surface molecules which are involved in signal transduction, cell-cell adhesion, and cellular activation. Association of CD81 with integrins appears to be functionally important in the regulation of cell adhesion and activation. Recent work with CD81-null mice indicates CD81 is important for development of humoral immune response. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml; 80ul labels 5x10e5 human Raji cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured human Raji cells were washed and incubated 45 minutes on ice with 80ul of C2433-10A at a concentration of 5ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed using a FACS. 3. Cells stained positive with a mean shift of 1.74 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice