C2432-01R-ML490
CD80 (CD80 Antigen, CD80 Molecule, Activation B7-1 Antigen, B-lymphocyte Activation Antigen B7, B7, BB1, CD28 Antigen Ligand 1 B7-1 Antigen, CD28LG, CD28LG1, Costimulatory Factor CD80, CTLA-4 Counter-receptor B7.1, LAB7, T lymphocyte Activation Antigen CD80) (MaxLight 490)
Size
£837.00
- SKU:
- C2432-01R-ML490
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Clone:
- 3H1689 (MEM-233)
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
- Extra Details:
- MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. CD80 is a member of the immunoglobulin superfamily having an extracellular domain bearing both a single Ig-v-like domain, a single Ig-c-like domain, a transmembrane sequence and a short cytoplasmic domain. Although the predicted molecular weight for human CD80 is ~33kD, the presence of multiple (8) potential N-glycosylation sites results in a migration corresponding to ~60kD. Human CD80 along with CD86 act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4 involved in T cell activation and proliferation. Although CD80 binds to the same receptors as CD86 it displays quite different characteristics in its avidity and binding kinetics. CD80 is expressed by activated B cells, and is important in B cell-T cell interactions, being a ligand of CD28 and CD152 (CTLA-4). CD80, also known as B7-1, a ~60kD type 1 trans-membrane protein expressed of macrophages, dendritic cells and activated B-cells. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: Non-reducing conditions Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice