C2431-14H
CD79A (B Cell Antigen Receptor Complex-associated Protein alpha Chain, Ig-alpha, MB-1 Membrane Glycoprotein, Membrane-bound Immunoglobulin-associated Protein, Surface IgM-associated Protein, IGA, MB1) (APC)
Size
£832.00
- SKU:
- C2431-14H
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Leu33-Arg143 at the extracellular domain of human CD79A expressed in NS0 cells.
- Clone:
- 12K42
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- CD79A, also known as Iga and MB-1, is a 44kD transmembrane glycoprotein in the immunoglobulin superfamily. It contains a single Ig-like domain in its extracellular domain (ECD) and one cytoplasmic immunoreceptor tyrosine-based activation motif (ITAM). Alternate splicing generates a short isoform with a 39aa deletion in the ECD. Heterodimers of CD79A and CD79B/IgB associate with a membrane bound immunoglobulin on the B cell surface to form the B cell antigen receptor complex (BCR). CD79A and CD79B are required for BCR-mediated signaling and consequently for the development and activation of B lineage cells. Within the ECD, human CD79A shares 57% amino acid sequence identity with mouse and rat CD79A. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells using human peripheral blood lymphocytes. Optimal dilutions to be determined by the researcher. Absorption: 620-650nm Emission: 660-670nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice