C2431-14A1-BIOTIN

CD79a (CD 79a, CD79A Antigen Immunoglobulin Associated alpha, CD79a Molecule Immunoglobulin Associated alpha, B Lymphocyte Specific MB1 Protein, B cell Antigen Receptor Complex Associated Protein alpha Chain, IGA, Ig alpha, Immunoglobulin Associated Alpha, Ly54, MB 1, MB 1 Membrane Glycoprotein, MB1, MB1 Membrane Glycoprotein, Membrane Bound Immunoglobulin Associated Protein, Surface IgM Associated Protein) (Biotin)

Size

£837.00

SKU:: C2431-14A1-BIOTIN
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa202-216 of human CD79a.
Clone:: HM57
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: CD79a, also known as mb-1, is a 45kD protein that is expressed by B lymphocytes during differentiation from early pre-B cell stage through to plasma cells. The CD79a molecule associates with CD79b (B29) to form a heterodimer that is non-covalently linked to surface immunoglobulin, forming the B-cell receptor (BCR) complex. The CD79a/CD79b heterodimers are also necessary for intracellular signalling following antigen-binding to surface immunoglobulin. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Membrane permeabilization is required. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from BALB/c mice. Positive control: Human tonsil Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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