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C2431-01H-ML490

CD79b (BCR-Ig beta) (MaxLight 490)

Size

£837.00

SKU:
C2431-01H-ML490
Purity:
Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Hamster
Immunogen:
CD79 alpha/CD79 beta heterodimer purified from WEH1231 B cells.
Clone:
HM79-11
Format:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Extra Details:
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. CD79 beta is expressed by B cells as part of the B cell receptor complex (immunoglobulin and the CD79 alpha/beta heterodimer). Mouse CD79 beta is expressed at the cell surface of pro-B cells prior to surface immunoglobulin and is expressed throughout B cell differentiation. CD79 beta is a B cell specific marker, valuable for the detection of B cells at all maturation stages. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100; Use 10ul of the recommended working dilution to label 10e6 cells or 100ul whole blood. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Source: Tissue culture supernatant Fusion Partners: Spleen cells from immunized Armenian hamsters were fused with cell of the mouse X63-Ag8.653 myeloma cell line NS1 Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Shipping Conditions:
Blue Ice