C2430-25C1
CD75 (Beta-galactoside alpha-2,6-sialyltransferase 1, Alpha 2,6-ST 1, B Cell Antigen CD75, CMP-N-acetylneuraminate-beta-galactosamide-alp ha-2,6-sialyltransferase 1, MGC48859, Sialyltransferase 1, SIAT1, ST6 beta-galactosamide alpha-2,6-sialyltranferase 1, ST6GAL1, ST6Gal I, ST6GalI, ST6N)
Size
£825.00
- SKU:
- C2430-25C1
- Application:
- WB
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant corresponding to aa27-406 from human CD75 expressed in NSO cells (P15907).
- Clone:
- 11n35
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- ST6GAL1 is a 47-49kD member of the glycosyltransferase 29 family of proteins. It is principally expressed in hepatocytes, placenta and skeletal muscle, and may be unique in its ability to generate Sia-a-2, 6-Gal structures on liver proteins. Human ST6GAL1 is a 406aa type II transmembrane protein that resides in Golgi membranes. It contains a short cytoplasmic segment (aa1-9) and an extended luminal domain (aa27-406) that possesses a phosphorylation site at Tyr369. ST6GAL1 undergoes BACE1-mediated cleavage to generate a 364aa, 52-53kD soluble form. This acts within the Golgi lumen to glycosylate proteins destined for secretion. There is one potential alternative start site at Met232. Over aa27-406, human ST6GAL1 shares 75aa identity with mouse ST6GAL1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice