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C2430-01N1

CD74 (CD74 Antigen, DHLAG, Gamma Chain of Class II Antigens, HLA Class II Histocompatibility Antigen gamma Chain, HLA DR Antigens Associated Invariant Chain, HLA DR gamma, HLADG, I-a Antigen Associated Invariant Chain, I-a Associated Invariant Chain, I-a gamma, I-i, Invariant Polpypeptide of Major Histocompatibility Class II Antigen Associated, La Associated Invariant Chain, La gamma, Major Histocompatibility Complex Class II Invariant Chain, MHC HLA DR gamma Chain, p33, Protein 41) (FITC)

Size

£620.00

SKU:
C2430-01N1
Application:
Flow Cytometry
Purity:
Purified by Protein G affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
B lymphoblastoid cell line HFB1.
Clone:
Bu45
Format:
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:
CD74 is expressed on MHC class II positive cells including B cells, a subset of activated T cells, monocytes, dendritic cells and by various types of carcinoma. Expression of CD74 is primarily intracellular with moderate expression at the cell surface of B cells and monocytes. The Bu45 antibody binds to an epitope within the extracytoplasmic domain of the CD74 molecule, which is distal from the C-terminus. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat-1:5; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice