C2428-41V

CD72 (B Cell Differentiation Antigen CD72, Lyb-2)

Size

£422.00

SKU:: C2428-41V
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Clone:: 10B1957 (Bu40)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: CD72 is a ~86kD cell surface homodimer expressed on B lymphocytes. CD72 is expressed on early B cell precursors, through to mature activated B cells, but is lost upon terminal differentiation to plasma cells. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Immunohistochemistry (paraffin): 1:50; Requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Use sodium citrate buffer, pH 6, for heat mediated antigen retrieval. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.