C2426-02H

CD71 (CD71 Antigen, p90, T9, Transferrin Receptor, TFR, TR, Transferrin Receptor Protein 1, TFR 1, TFR1, TFR-1, TFRC, TRFR)

Size

£576.00

SKU:: C2426-02H
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Pre-B cell line NALM-6
Clone:: MEM-75
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: CD71 (transferrin receptor) is a type II transmembrane glycoprotein expressed as homodimer in erythroid blood cell line and in activated leukocytes. Upon binding of holotransferrin (complex of transferrin and iron ions), CD71 is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with CD71 and recycles to the cell surface, where it is released upon exposure to normal pH. CD71 is also involved in uptake of non-transferrin bound iron. Applications: Suitable for use in Flow Cytometry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.