C2425-01M

CD70 (Tnfsf7, CD27 Ligand) (Biotin)

Size

£557.00

SKU:: C2425-01M
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human WM-1 (Waldenström's macroglobulinemia) cell line.
Clone:: 7H173
Format:: Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 5% glycerol, 0.2% BSA, 0.04% sodium azide Labeled with Biotin.
Extra Details:: Human CD70 is not present on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. CD70 is a ligand for CD27 and it is also a member of the tumor necrosis factor gene family. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Functional Application: Antibody inhibits T cell proliferation induced by dendritic cells. Recommended Dilutions: Flow Cytometry: 10ug/ml. Use 80ul to label 5x10e5 Raji cells. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-78R2: IgG1 Murine Negative Control (Biotin) Flow Cytometry Protocol: 1. 5x10e5 cultured Raji cells were washed, then incubated 45 minutes on ice with 80ul of C2425-01M at 10ug/ml. 2. Cells were then washed twice and incubated for 45 minutes with S7973-88P: Streptavidin (PE). 3. Cells were washed three times, fixed and analyzed by FACS. 4. Cells stained with a mean shift of 1.32 log10 fluorescent units when compared to I1904-78R2 IgG1 Murine Negative Isotype Control (Biotin) at a similar concentration. 5. Binding was blocked when pre-incubated for 10 minutes with 20ul of 0.5mg/ml of anti-CD70. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice