C2425-01C
CD70 (Tnfsf7, CD27 Ligand)
Size
£889.00
- SKU:
- C2425-01C
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Gln45-Pro193 from human CD27 Ligand expressed in NS0 cells.
- Clone:
- 7H76
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- CD27 Ligand is a member of the tumor necrosis factor superfamily (TNFSF7) and is also designated CD70. CD27 Ligand is a type II transmembrane protein expressed by activated T and B cells. It binds to CD27 on mature T cells and a subset of thymocytes. CD27 Ligand induces the proliferation of costimulated T cells and enhances the generation of cytolytic T cells. Applications: Suitable for use in Direct ELISA, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 8-25ug/ml labels immersion fixed human peripheral blood mononuclear cells Western Blot: 1ug/ml using recombinant human CD27 Ligand/TNFSF7. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice