C2424-01A
CD69 (Biotin)
Size
£557.00
- SKU:
- C2424-01A
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- IL-2 activated human NK cells.
- Clone:
- 7H192
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
- Extra Details:
- Human CD69 is expressed by platelets and mature thymocytes and appears rapidly after activation on T cells, B cells and NK cells. This pattern of cellular CD69 expression has suggested a lymphocyte activation role. Functional studies with antibodies to CD69 also support an activation role for CD69. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HEL92.1 cells were washed and incubated 45 minutes on ice with 80ul of C2424-01A at a concentration of 10ug/ml. 2. Cells were washed twice and incubated with S7973-88P, Streptavidin-PE 3. Washed three times, fixed and analyzed by FACS. 4. Cells stained positive with a mean shift of 1.38 log10 fluorescent units when compared to a mouse IgG2a/Biotin negative control. 5. Binding was 92% blocked when reagent was pre-incubated 10 minutes with 20ul of 0.5mg/ml C2424-01. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice