C2424-01
CD69
Size
£479.00
- SKU:
- C2424-01
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- IL-2 activated human NK cells
- Clone:
- 7H192
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human CD69 is expressed by platelets and mature thymocytes and appears rapidly after activation on T cells, B cells and NK cells. This pattern of cellular CD69 expression has suggested a lymphocyte activation role. Functional studies with antibodies to CD69 also support an activation role for CD69. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HEL cells were washed and incubated 45 minutes on ice with 80ul of C2424-01 at 5ug/ml. 2. Cells were washed twice and incubated with 2º reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.44 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice