C2423-05N6
CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin, Scavenger Receptor Class D Member 1, SCARD1) (PE)
Size
£940.00
- SKU:
- C2423-05N6
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat spleen cells
- Clone:
- 10B1909 (ED1)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization. CD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37408D (354aa) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. The antigen is expressed on most macrophages populations, as well as on monocytes and is considered as a pan-macrophage marker in the rat. It is expressed predominantly on the lysosomal membrane and lightly on the cell surface. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat-1:10; 10ul labels 1x10e6 cells in 100ul. Membrane permeabilization is required Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C before and after reconstitution. DO NOT FREEZE! Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute lyophilized powder with sterile ddH2O prior to use and store remainder as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice