C2423-05F
CD68 (MACROSIALIN, Macrophage antigen, SCARD1, Scavenger receptor class D member1) (FITC)
Size
£429.00
- SKU:
- C2423-05F
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Purified Concanavalin A acceptor glycoprotein from P815 cell line
- Clone:
- 6A326 (FA-11)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).
- Extra Details:
- CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homolog of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (LAMP) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization. CD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37408D (354 amino acids) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required for this application. Optimal dilution determined by the researcher Recommended Negative Control: I1904-82E1: IgG2a, Rat Negative Control (FITC) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice