C2423-05B
CD68 (Macrosialin, Macrophage Antigen, SCARD1, Scavenger Receptor Class D Member 1) (FITC)
Size
£806.00
- SKU:
- C2423-05B
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Cell suspensions of human lymph node.
- Clone:
- Ki-M7
- Format:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD68 is an integral membrane glycoprotein of ~110kD also known as Macrosialin or Gp110. CD68 is predominantly expressed on the intracellular lysosomes of macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including Acute Myeloid Leukemia. In immunohistochemical studies, CD68 can be used to aid in the identification of blastic NK lymphomas, some B cell lymphomas, and to help diagnose disorders relating to macrophage abnormalities, including malignant histocytosis and Gaucher’s disease. Applications: Suitable for use in Flow Cytometry. Although not tested, this clone has also been reported as being suitable for use in immunoprecipitation studies. Other applications not tested. Recommended Dilution: Flow Cytometry: Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required for this application. Optimal dilution determined by the researcher Recommended Negative Controls: Mouse IgG1 Negative Control: FITC Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice