C2423-02A

CD68 (Macrophage antigen CD68 (microsialin), GP110, SCARD1, Scavenger receptor class D member1)

Size

£384.00

SKU:: C2423-02A
Application:: IHC-Fr
Purity:: Supernatant
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Gaucher cells. Cellular Localization: Cytoplasmic
Clone:: 5G61
Format:: Supplied as a liquid in 0.09% sodium azide.
Extra Details:: CD68, also called Scavenger Receptor D1 (SR-D1), is a type I transmembrane glycoprotein. Macrosialin is the murine homologue of the human macrophage glycoprotein CD68, both are members of the lysosomal-associated membrane protein (lamp) family which are located predominantly within the cells and can be detected by flow cytometry using cell permeablization. CD68 is a 110kD transmembrane (Type I) glycoprotein consisting of a polypeptide of 37408D (354 amino acids) predicted from the cDNA sequence that is highly expressed by human monocytes and tissue macrophages. CD68 plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes, lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells. Applications: Suitable for use in Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Tonsil Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice