C2419-06-HRP
CD64 (FcgRI) (HRP)
Size
£940.00
- SKU:
- C2419-06-HRP
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human monocytes.
- Clone:
- 10.1
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
- Extra Details:
- Human CD64 is a high affinity receptor for monomeric human IgG1 and IgG3 which is expressed on macrophages, monocytes, and gamma interferon induced neutrophils. CD64 plays an important role in clearance of immune complexes and in antibody dependent cytoxicity. CD64, also known as FcgRI, is a 71kD type I transmembrane protein. It is expressed exclusively on monocytes and macrophages and binds monomeric human IgG with high avidity. CD64 is thought to play a role in the antibody-dependent cytotoxicity (ADCC) reactions, phagocytosis and mediates the release of IL-1, IL-6 and TNF-alpha. INF-gamma increases the expression of CD64 and induces its expression on neutrophils which do not usually express CD64. Glucocorticoids have also been reported to increase this receptor. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice