C2418-09
CD63
Size
£633.00
- SKU:
- C2418-09
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Designated as a lysosomal membrane protein characterized as an activation dependent platelet surface antigen which is diversely distributed on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma.
- Clone:
- 2Q1430 (RFAC4)
- Format:
- Supplied as a liquid in PBS, 0.1% BSA, 0.09% sodium azide
- Extra Details:
- CD63 is a multi-pass, lysosomal membrane protein belonging to the tetraspan (or tetraspanin) transmembrane protein family. The structure of CD63 is comprised of 4 domains spanning the membrane; two unequally sized extracellular loops and two, short domains within the cytoplasm, which are thought to participate in signal transduction for some cell types. CD63 also contains a characteristic lysosomal targeting motif, GYWVM, within its C-terminal sequence. Although the exact function of CD63 has yet to be elucidated, it has been implicated as having a role in growth regulation and to be involved in the initial stages of melanoma tumor progression. Expression of CD63 has been observed in dysplastic nevi, hematopoietic cells, radial growth phase primary, and in tissue macrophages. Studies have suggested a role for CD63 in macrophage infection from CCR5-mediated HIV-1. Loss of CD63 expression has been observed within in a patie4nt suffering from a rare, genetic disorder known as Hermansky-Pudlak syndrome (HPS). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: Detects human CD63 in blotting of CD63 antigen from solubilized platelet proteins. Immunohistochemistry: Formalin-fixed paraffin embedded tissue sections. HIER and proteolytic pretreatment may not be necessary. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice