C2418-02L
CD63 (CD63 Antigen, Cd63 Molecule, Mast Cell Antigen AD1, MGC72893)
Size
£358.00
- SKU:
- C2418-02L
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- RBL-2H3 rat basophilic leukemia cell line.
- Clone:
- AD1
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Extra Details:
- CD63 is an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, which is translocated to the cell surface upon activation of platelets, and therefore a potential indicator of platelet activation. Rat CD63 is expressed on mast cells, and studies using clone AD1, have shown that it is located near to FcER1, and that clone AD1 partially inhibits IgE-mediated histamine release. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: paraffin sections Flow Cytometry: 1:10-1:200 Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. IHC Positive Control Tissue: Mast cells or basophils. Hybridoma: X63-Ag8-653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice