C2418-02K
CD63
Size
£889.00
- SKU:
- C2418-02K
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Met1-Met238 from mouse CD63, expressed in HEK293 cells.
- Clone:
- 9H90
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- CD63, also known as LAMP-3 or ME491 (melanoma- associated antigen), is a member of the tetraspanin superfamily of protein trafficking proteins. CD63 is ubiquitously expressed and found in late endocytic vesicles, but following cell activation is also present on the plasma membrane. CD63 interacts with other membrane proteins or adaptors to regulate activities such as adhesion, migration and degranulation. The 238aa CD63 migrates at 30-60kD, depending on post-translational modification. Extracellular regions of mouse CD63 share 94% and 67% aa identity with rat and human CD63, respectively. Applications: Suitable for use in Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml detects CD63 in perfusion fixed frozen sectoins of mouse kidney Flow Cytometry: 0.25ug/ml detects CD63 in HEK293 Human Cell Line Transfected with Mouse CD63 and eGFP Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice