C2418-02F

CD63 (CD63 antigen, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, Melanoma-associated antigen ME491, MLA1, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, TSPAN30, Tspan-30) (PE)

Size

£735.00

SKU:: C2418-02F
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human eosinophils
Clone:: 7H144
Format:: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with R-Phycoerythrin (PE).
Extra Details:: Human CD63 is expressed on activated platelets and also on monocytes and macrophages. CD63 contains four hydrophobic transmembrane regions and is a member of the tetraspan family. CD63 appears to be involved with cell adhesion and associates with VLA-3 and VLA-6 integrins. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (or use 1.6ul concentrated stock per 5x10e5 cells) Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured HPB-MLT cells were washed and incubated 45mins on ice with 80ul of C2418-02F at a 1:50 dilution (20ug/ml). 2. Cells were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.64 log10 fluorescent units when compared to a mouse IgG1/PE negative control at a similar concentration. 4. Binding was blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml anti-CD63 antibody (Cat.# C2418-02C). Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Shipping Conditions:: Blue Ice