C2418-02D

CD63 (CD63 antigen, Granulophysin, LAMP-3, Lysosomal-associated membrane protein 3, ME491, Melanoma-associated antigen ME491, MLA1, Ocular melanoma-associated antigen, OMA81H, Tetraspanin-30, TSPAN30, Tspan-30) (Biotin)

Size

£607.00

SKU:: C2418-02D
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human eosinophils.
Clone:: 7H144
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Extra Details:: Human CD63 is expressed on activated platelets and also on monocytes and macrophages. CD63 contains four hydrophobic transmembrane regions and is a member of the tetraspan family. CD63 appears to be involved with cell adhesion and associates with VLA-3 and VLA-6 integrins. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 20ug/ml labels 5x10e5 cultured HPB-MLT human tumor cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and preincubated 5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding). 2. After which they were incubated 45 minutes on ice with 80ul of C2418-02D at 20ug/ml. 3. Cells were washed twice and incubated with S7973-88P: Streptavidin (PE), after which they were washed three times, fixed and analyzed using FACS. 4. Cells stained positive with a mean shift of 1.65 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of C2418-02C. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice