C2418-02-HRP
CD63 (HRP)
Size
£940.00
- SKU:
- C2418-02-HRP
- Application:
- IP
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HPB-ALL cell line.
- Clone:
- MEM-259
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish peroxidase (HRP).
- Extra Details:
- CD63 is a lysosomal granule-membrane glycoprotein and has been detected on the surface and in the cytoplasma of a variety of cells, particularly in platelets; surface expression indicates lysosomal secretion. CD63, present only in azurophil granules of non-stimulated neutrophils, is strongly expressed at the surface of neutrophils after activation showing that CD63 is a good marker for granule release; this phenomenon has been also reported for basophils. CD63 is present in cell line PHA blasts, K562, U937, KG1a, RAJI. Both surface and cytoplasmic reactions are reported. CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma. Applications: Suitable for use in Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice