C2418-02-BIOTIN
CD63 (Biotin)
Size
£940.00
- SKU:
- C2418-02-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- HPB-ALL cell line.
- Clone:
- MEM-259
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- CD63 is a lysosomal granule-membrane glycoprotein and has been detected on the surface and in the cytoplasma of a variety of cells, particularly in platelets; surface expression indicates lysosomal secretion. CD63, present only in azurophil granules of non-stimulated neutrophils, is strongly expressed at the surface of neutrophils after activation showing that CD63 is a good marker for granule release; this phenomenon has been also reported for basophils. CD63 is present in cell line PHA blasts, K562, U937, KG1a, RAJI. Both surface and cytoplasmic reactions are reported. CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunofluorescence. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice