C2414-92-ML650

CD62E (LECAM, E-Selectin, Endothelial Leukocyte Adhesion Molecule) (MaxLight 650)

Size

£837.00

SKU:: C2414-92-ML650
Purity:: Purified IgG.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: 3H1606
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Extra Details:: MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. Recommended Secondary Reagents: F(ab')2 rabbit anti-mouse IgG HRP, F(ab')2 rabbit anti mouse IgG: RPE conjugate Recommended Negative Controls: Mouse IgG2a Negative Control Applications: Flow Cytometry: 1:20 Immunohistochemistry: (frozen)- 0.5-2ug/ml Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.