C2414-91L
CD62E (CD62 antigen-like family member E, CD62E, ELAM, ELAM1, ELAM-1, Endothelial leukocyte adhesion molecule 1, ESEL, E-selectin, LECAM2, Leukocyte-endothelial cell adhesion molecule 2)
Size
£479.00
- SKU:
- C2414-91L
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- TNF activated HUVEC
- Clone:
- HAE-1f
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
- Extra Details:
- Human CD62E (E-selectin) is an adhesion molecule expressed on activated endothelium and facilitates recruitment of leukocytes to sights of acute inflammation and immunological events. CD62E has affinity to LewisX (1). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Cultured human umbilical cord vein endothelial cells (HUVEC) were stimulated 4 hours with 10ng/ml Phorbol 12-Myristate 13-Acetate. Five x 105 cells per tube were harvested, washed and incubated 45 min on ice with 80ul of anti-CD62E antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.61 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice