C2414-10B

CD61 (Glycoprotein IIIa, Integrin beta3)

Size

£806.00

SKU:: C2414-10B
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human platelet membrane fraction
Clone:: SAP
Format:: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Extra Details:: Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 5-20ug/ml showed positive immunostaining for Integrin 3 (CD61) in HuVec cells fixed with 4% paraformaldehyde followed by permeabilization with 0.25% Triton X-100. Western Blot: Detects Integrin 3 (CD61) in WM852 and 1205Lu whole cell extracts (1,2). Immunohistochemistry: Detects Integrin 3 (CD61) in malignant melanoma sections (1). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.