C2413-22B

CD59 (16.3A5, 1F5, CD59a, EJ16, EJ30, EL32, G344, 20kD Homologous Restriction Factor, HRF20, HRF-20, Ly-6-like Protein, Lymphocytic Antigen CD59/MEM43, MAC Inhibitory Protein, MAC-IP, MEM43 Antigen, Membrane Attack Complex (MAC) Inhibition Factor, MACIF, Membrane Inhibitor of Reactive Lysis, MIRL, MIC11, MIN1, MIN2, MIN3, MSK21, p18-20, Protectin, Surface Anitgen Recognized by Monoclonal Antibody 16.3A5, T cell Activating Protein)

Size

£576.00

SKU:: C2413-22B
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Thymocytes and T lymphocytes
Clone:: MEM-43/5
Format:: Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Extra Details:: CD59 (protectin) is a small (18-20kD) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml, Non-reducing conditions Immunohistochemistry (paraffin): 5ug/ml Flow Cytometry: 0.5-4ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Mouse spleen Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.