C2413-21R
CD59 (MACIF, MIRL, Protectin) (PE)
Size
£704.00
- SKU:
- C2413-21R
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human K562 tumor cells
- Clone:
- 7H165
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Lableled with PE.
- Extra Details:
- Human CD59 is a GPI anchored molecule that protects cells from complement-mediated lysis (1). Studies are progressing to determine the regulatory mechanism for CD59 expression (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 (~80ul/test) Optimal dilutions to be determined by the researcher. FACS Protocol: 1. Five x 10e5 cultured HPB-MLT cells were washed and preincubated ~5 minutes with 20ul of 250mg/ml human IgG (to block nonspecific binding). 2. After which they were incubated 45 minutes on ice with 80ul of C2413-21R at a dilution of 1:50. 3. Cells were washed three times, fixed and analyzed using a BD FACS Calibur. 4. Cells stained positive with a mean shift of 2.81 log10 fluorescent units when compared to a Mouse IgG1/R-PE negative control at a similar concentration. 5. Binding was 92% blocked when pre incubated 10 minutes with 20ul of 0.5mg/ml C2413-21N. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice