C2413-21P
CD59 (MACIF, MIRL, Protectin) (Biotin)
Size
£557.00
- SKU:
- C2413-21P
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human K562 tumor cells
- Clone:
- 7H165
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol.
- Extra Details:
- Human CD59 is a GPI anchored molecule that protects cells from complement-mediated lysis (1). Studies are progressing to determine the regulatory mechanism for CD59 expression (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml labels 5x10e5 cultured human HPB-MLT cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and preincubated 5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding). 2. After which they were incubated 45 minutes on ice with 80ul of C2413-21P at 10ug/ml. 3. Cells were washed twice and incubated with S7973-88P: Streptavidin (PE), after which they were washed three times, fixed and analyzed using FACS. 4. Cells stained positive with a mean shift of 1.9 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of 0.5 mg/ml anti-CD59 antibody. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice