C2412-06J
CD58 (LFA-3)
Size
£479.00
- SKU:
- C2412-06J
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human cytotoxic T cells
- Clone:
- 7H219
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human CD58 is a cell surface glycoprotein that functions as an adhesion molocule and mediates costimulatory activity through its ligand CD2, which is expressed on T and natural killer cells (1,2). CD58 has wide cellular distribution on both hemopoietic and non-hemopoietic cells with strong expression on macrophages and memory T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured Nalm-6 cells were washed after which they were incubated 45 minutes on ice with 80ul of C2412-06J at 10ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse after which they were washed three times, fixed and analyzed by BD FACS Calibur. 3. Cells stained positive with a mean shift of 1.46 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice