C2412-06A
CD58 (LFA-3)
Size
£371.00
- SKU:
- C2412-06A
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human erythrocytes.
- Clone:
- BRIC-5
- Format:
- Supplied as a liquid in Tris buffered glycine, 0.09% sodium azide.
- Extra Details:
- CD58, or LFA-3, is a membrane glycoprotein of 55-70kD. It occurs in two forms, one transmembrane with a cytoplasmic domain, the other form anchored in the membrane via a glycosylphosphatidylinositol tail. The complete amino acid sequence of both forms has been deduced from cDNA. It is heavily N-glycosylated. CD58 is a cell adhesion molecule which plays a critical role in facilitation of antigen specific recognition through interaction with CD2 on T lymphocytes. It is a member of the immunoglobulin superfamily of molecules. CD58 has a wide tissue distribution, being present on erythrocytes, platelets, monocytes, a subset of lymphocytes, bone marrow cells, epithelium and endothelial cells. There are approximately 5000 CD58 molecules on each erythrocyte. There is reduced expression of CD58 on hemopoietic cells in individuals with paroxysmal nocturnal hemoglobinuria. BRIC-5 was produced in response to erythrocytes. The functional affinity of BRIC-5 binding to erythrocytes is 4x10e8M-1. It reacts by immunoblotting to non-reduced erythrocyte membranes. BRIC-5 is an indirect hemagglutinin. The antigen on erythrocytes is Pronase sensitive. BRIC-5 inhibits T cell rosetting. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10. Use 10ul to label 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice