C2411-23D

CD57 (Beta-1,3-glucuronyltransferase 1, CD57, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, GLCATP, GlcAT-P, GLCUATP, GlcUAT-P, Glucuronosyltransferase P, HNK1, HNK-1, LEU7, NK1, NK-1, UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase)

Size

£479.00

SKU:: C2411-23D
Purity:: Purified by size exclusion chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human peripheral blood mononuclear cells
Clone:: NK-1
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
Extra Details:: Human CD57 originally called HNK-1 is a glycoprotein found on 15-20 percent of PBL’s, including 60 percent of NK cells, and a subset of T cells (1). The immune regulation role of CD57 postive PBL’s expressing high levels of CD8 is being investigated (2). Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Fixed cells or tissues. Flow Cytometry: 5x10e5 cultured Jurkat human tumor cells were washed and incubated 45 min on ice with 80ul of anti-CD57 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC; after which they were washed three times, fixed and analyzed by FACS. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.