C2411-23C

CD57 (CD57 Antigen, Beta-1,3-glucuronyltransferase 1, B3GAT1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, Glucuronosyltransferase P, GLCATP, GlcAT-P, GlcUATP, GlcUAT-P, HNK1, HNK-1, LEU7, LEU7 Antigen, NK1, NK-1, UDP-GlcUA Glycoprotein beta-1,3-glucuronyltransferase)

Size

£550.00

SKU:: C2411-23C
Purity:: Purified by Ion Exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human neuroblastoma cells
Clone:: 3H1574 (TB01)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: CD57 (HNK-1) is an antigen associated with natural killer cells. It is also found in T-cell subsets and B cell subsets. This antibody is similar to HNK-1 and Leu-7. It detects CD 57 antigen (110kD) on the surface of NK cells in germinal centers, and almost 20% of peripheral blood mononuclear cells in adult. It may cross react to myelin-associated glycoprotein of peripheral nerves and prostate surface antigen. This antibody may be useful for the study of NK cell-mediated cytotoxity and functional properties of NK cells and T-cell subsets. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100; 10ul labels 10e6 cells in 100ul Immunohistochemistry (frozen): 1:10-1:100 Immunohistochemistry (paraffin): 1:10-1:50 Does not require antigen retrieval using heat treatment prior to staining sections but sodium citrate buffer may enhance staining. Optimal dilutions to be determined by the researcher. Hybridoma: P3.X63 Ag8.653 myeloma cells with spleen cells from BALB/c mice. Recommended Secondary Antibody: I1905-01D: IgM (AP) Pab. Gtx Mo Recommended Negative Control: I1905-26C: IgM Murine Negative Control Recommended Positive Control (IHC): Human Tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice