C2410-55D
CD56 (NCAM-1, Neural Cell Adhesion Molecule) (Biotin)
Size
£557.00
- SKU:
- C2410-55D
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human CD56 (truncated domains 1-4 only)
- Clone:
- 7H153
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
- Extra Details:
- Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses. Applications: Suitable for use in Flow Cytometry, Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml (80ul) labels 5x10e5 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared from human peripheral blood mononuclear cells per tube were washed and preincubated for ~5 minutes with 20ul of 300ug/ml human IgG (to block nonspecific binding). 2. They were for incubated 45 minutes on ice with 80 ul of C2410-55D at 10ug/ml. 3. Cells were washed twice and incubated with S7973-88P, after which they were washed twice, fixed and analyzed by FACS. 4. A net 24% sub population of the cells stained positive with a mean shift of 1.38 log10 fluorescent units when compared to a Mouse IgG1/Biotin isotype control. Binding was blocked when cells were preincubated with 20ul of 0.5mg/ml C2410-55C. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice