C2410-55B

CD56 (NCAM-1, Neural Cell Adhesion Molecule) (Biotin)

Size

£704.00

SKU:: C2410-55B
Purity:: Purified by Protein A affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human retinoblastoma tumor tissue, membrane fraction
Clone:: 7H185
Format:: Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Extra Details:: Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): acetone fixation Flow Cytometry: 10ug/ml will label 10e5 cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared human peripheral white blood cells were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80ul of C2410-55B at a concentration of 10ug/ml. 2. Cells were washed twice and incubated with Streptavidin (PE) (Catalog #S7973-88P). 3. After which they were washed three times, fixed and analyzed using by FACS. A net 15% sub population of the cells stained positive with a mean shift of 0.94 log10 fluorescent units when compared to a Mouse IgG1 (Biotin) negative control at a similar concentration. 4. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD56 antibody (Catalog #C2410-55A). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice