C2410-50D
CD55 (DAF, Decay Accelerating Factor) (PE)
Size
£479.00
- SKU:
- C2410-50D
- Purity:
- Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Clone:
- 143-30
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
- Extra Details:
- CD55, also known as decay accelerating factor (DAF), is a 64-73kD GPI-anchored single chain glycoprotein that is expressed on all cells in contact with serum, including all hematopoietic cells and the vascular endothelium. A soluble form of CD55 is present in plasma and body fluids. CD55 is a member of the regulator of complement activity (RCA) family of proteins and is thought to play a role in the protection of cells from damage by autologous complement by preventing the amplification steps of the complement cascade. CD55 expression increases upon T cell activation and antibodies to CD55 are mitogenic in the presence of phorbol esters.1-5 Applications: • Flow cytometry • Immunohistochemistry (frozen sections) Recommended Dilution: Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice